![]() The 2-D reference map with pH value from 3.5 to 9.0 was synthesized by using the ImageMaster analysis software. After 2-DE, several visually identical spots between the different pH range 2-D gel pairs were cut from the gels and confirmed by mass spectrometry and used as landmarks for computer analysis. ![]() In order to reveal more protein spots, immobilized pH gradient strips (24 cm) of broad range of pH 3-10 and the narrower range of pH 6-9, as well as different overlapping narrow range pH immobilized pH gradient (IPG) strips, including 3.5-4.5, 4.0-5.0, 4.5-5.5, 5.0-6.0 and 5.5-6.7, were used. To extend the analysis of the proteome of human spermatozoa and establish a 2-D gel electrophoresis (2-DE) reference map of human spermatozoal proteins in a pH range of 3.5-9.0. Li, Ling-Wei Fan, Li-Qing Zhu, Wen-Bing Nien, Hong-Chuan Sun, Bo-Lan Luo, Ke-Li Liao, Ting-Ting Tang, Le Lu, Guang-Xiu These data will support the genome annotation process and provide a basis on which to undertake comparative proteomic studies.Įstablishment of a high-resolution 2-D reference map of human spermatozoal proteins from 12 fertile sperm-bank donors. Seventeen hypothetical or conserved hypothetical ORFs were shown to code for true gene products. Using MALDI-TOF-MS we identified 205 spots representing 173 different proteins with a calculated average of 1.18 proteins/gene. Here, we present a 2-D reference map for H. Herbaspirillum seropedicae is an endophytic diazotroph associated with economically important crops such as rice, sugarcane, and wheat. A two-dimensional proteome reference map of Herbaspirillum seropedicae proteins.Ĭhaves, Daniela Fojo Seixas Ferrer, Pércio Pereira de Souza, Emanuel Maltempi Gruz, Leonardo Magalhães Monteiro, Rose Adele de Oliveira Pedrosa, Fábio ![]()
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